Dr B's Cancer Research - Genetics, Microbiology, Immunology
Samples of oral tissue from patients with oral mucosal lesions and throat washings from healthy individuals were screened for HHV-6 DNA using nested PCR. The appropriate amplified fragments were confirmed by non-radioactive hybridization with a HHV-6 specific oligonucleotide probe. 

DNA was extracted from: 
  • fresh oral biopsies from 9 patients from India with squamous cell carcinoma (SCC)
  • paraffin-embedded tissues of 5 Malaysian patients (2 with Lichen Planus and 3 with Leukoplakia), obtained from the Institute for Medical Research (IMR)
  • throat washings of 22 healthy Malaysian individuals

Polymerase Chain Reaction (PCR)
Honors Dissertation